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White Labs WLN4100 Ultra Ferm - Enzymes (10mL)
White Labs WLN4100 Ultra Ferm - Enzymes (10mL)
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ULTRA-FERM is a liquid amyloglucosidase (highly concentrated) from selected classical strain of Aspergillus niger.
A suitable substrate for saccharification is a liquefied starch with a dextrose equivalent (DE) between 10 and 20. A higher DE than 20 might reduce the saccharification efficiency. The concentration of the substrate will also influence the saccharification efficiency. A concentration of 20-25% is a general practice. ULTRA-FERM dosage, for given reaction time, depends on the amount of glucose requested. It is usually recommended to add 400 ml per ton of starch for a fast hydrolysis. Possible pH range of use is from 3.5 to 5.5 with optimal between 4.0 and 5.3.
Optimal temperature is 60°C. Amyloglucosidase activity is completely destroyed when the saccharified liquor is held at 85°C for 10 minutes. As an example: a yield of 94% glucose can be obtained after 40 hours with 36000 AGI per kg dry matter; after 72 hours with 17000 AGI/kg and after 85 hours with 13000 AGI/kg, at pH 4.5 with temperature 60°C.
Usage Directions:
Applications:
A suitable substrate for saccharification is a liquefied starch with a dextrose equivalent (DE) between 10 and 20. A higher DE than 20 might reduce the saccharification efficiency. The concentration of the substrate will also influence the saccharification efficiency. A concentration of 20-25% is a general practice. ULTRA-FERM dosage, for given reaction time, depends on the amount of glucose requested. It is usually recommended to add 400 ml per ton of starch for a fast hydrolysis. Possible pH range of use is from 3.5 to 5.5 with optimal between 4.0 and 5.3.
Optimal temperature is 60°C. Amyloglucosidase activity is completely destroyed when the saccharified liquor is held at 85°C for 10 minutes. As an example: a yield of 94% glucose can be obtained after 40 hours with 36000 AGI per kg dry matter; after 72 hours with 17000 AGI/kg and after 85 hours with 13000 AGI/kg, at pH 4.5 with temperature 60°C.
Usage Directions:
- In the brewhouse, the recommended application dosage of Ultra-Ferm is 0.8 to 3.2L per ton of starch
- It can be added at the beginning of the mash-in
- The recommended application dosage is 1.5 to 2.5 milliliters per barrel of beer, applied as the fermenter is being filled
- Ultra-Ferm optimal pH is between 3.5 and 5.5
- Temperature should not exceed 60°C
- Amyloglucosidase activity is completely destroyed when the wort is held at 85°C for 10 minutes
Applications:
- To reduce saccharification times
- To saccharify unmalted cereal brews
- To increase attenuation
- Brewers often want to produce light beers or dietetic beers
- In these cases the brewer wants a controlled or complete hydrolysis of starch and dextrins to fermentable glucose
- Traditional brewing methods permit only 75 to 80% hydrolysis of starch present in the grain raw material
- Ultra-Ferm amyloglucosidase permits total hydrolysis of dextrins to fermentable glucose, from all types of starch
- After starch liquefaction by alpha-amylase, dextrins are hydrolysed in glucose by amyloglucosidase, before alcoholic fermentation
- Ethanol production is faster and yield is higher
- The recommended saccharification temperature is 60°C
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